A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

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A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the prese...

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Sorbitol Dehydrogenase Activity in Diabetes Mellitus and Cataract Patients

Accumulation of sorbitol in the pathogenesis of cataract in susceptible subjects has long been an issue. Sorbitol dehydrogenase (SDH) activity in 25 diabetes mellitus (DM) patients and 15 patients having cataract was investigated using 10 subjects without diabetes or cataract as the control. Results of fasting plasma glucose assay done on the DM patients showed a mean range of 64 – 144mg/dl in ...

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inhibition of aldose reductase and red blood cell sorbitol accumulation by extract of capparis spinosa

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Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana.

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1983

ISSN: 0021-9746

DOI: 10.1136/jcp.36.6.697